p irf3 (Cell Signaling Technology Inc)
Cell Signaling Technology Inc is a verified supplier 
Cell Signaling Technology Inc manufactures this product 
96
Structured Review
Cell Signaling Technology Inc
p irf3
P Irf3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 633 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+p+irf3+ser396/Phospho-IRF-3+(Ser396)+Rabbit+mAb/pmc12813888-103-34-36
Average 96 stars, based on 633 article reviews
P Irf3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 633 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+p+irf3+ser396/Phospho-IRF-3+(Ser396)+Rabbit+mAb/pmc12813888-103-34-36
Average 96 stars, based on 633 article reviews
p irf3 - by Bioz Stars,
2026-10
96/100 stars
Images
1) Product Images from "On-demand mild photothermal cascade platform reprogramming mitochondrial immunity for tendon rejuvenation"
Article Title: On-demand mild photothermal cascade platform reprogramming mitochondrial immunity for tendon rejuvenation
Journal: Bioactive Materials
doi: 10.1016/j.bioactmat.2026.01.004
Figure Legend Snippet: Schematic illustration of the ROS-responsive on-demand mild photothermal cascade platform for tendon rejuvenation in Achilles tendinopathy. The platform strategically modulates the mitochondrial-cGAS-STING-IRF3/NF-κB signaling axis and induces heat shock protein 70 (HSP70) expression to attenuate oxidative stress and cellular senescence within tendon stem/progenitor cells (TSPCs). Consequently, it promotes tenogenic differentiation while abrogating aberrant osteogenic/chondrogenic lineage commitment. This cascade effect ultimately mitigates heterotopic ossification, enhances structural tendon regeneration, restores biomechanical function, and alleviates pain. Nanoparticle nomenclature: LA-NPs and TPA-TCNQ-NPs denote nanoparticles encapsulating LA or TPA-TCNQ individually; LT-NPs refers to the composite formulation consisting of a mixture of LA-NPs and TPA-TCNQ-NPs; and LT-NPs-NIR represents the LT-NPs mixture following 808 nm near-infrared (NIR) irradiation to activate the photothermal response and controlled payload release.
Techniques Used: Expressing, Formulation, Irradiation
Figure Legend Snippet: LT-NPs-NIR regulate the mtDNA-STING-IRF3/NF-κB pathway in macrophages. (A) Principal component analysis (PCA) of transcriptomic data from RAW 264.7 cells under different treatments. (B) Volcano plots comparing H 2 O 2 vs. Control (left) and LT-NPs-NIR vs. H 2 O 2 (right). (C) Heatmap of differentially expressed genes (DEGs) with hierarchical clustering. (D, E) Gene Ontology (GO), KEGG, and Gene Set Enrichment Analysis (GSEA) for H 2 O 2 vs. Control (D) and LT-NPs-NIR vs. H 2 O 2 (E). (F) Multi-SIM imaging showing mtDNA (magenta) and the mitochondrial outer membrane protein TOMM20 (green), with quantification of their colocalization. (G) Western blot analysis of key proteins in the cGAS-STING-NF-κB axis. Scale bar: 5 μm (F). Significance: ns, not significant; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.
Techniques Used: Control, Imaging, Membrane, Western Blot
Figure Legend Snippet: LT-NPs-NIR attenuate oxidative stress, preserve mitochondrial integrity, and suppress senescence in TSPCs by inhibiting the mtDNA-STING-NF-κB axis. (A) Schematic of the experimental design. (B) TSPC proliferation assessed by CCK-8 assay. (C, E) Immunofluorescence staining and quantification of HSP70 (n = 3). (D, F) Mitochondrial membrane potential (ΔΨm) visualized by JC-1 staining (red: high potential; green: low potential) and quantification. (G) Multi-SIM of mtDNA (magenta) and TOMM20 (green) with colocalization analysis. (H) Western blot of cGAS-STING-IRF3-NF-κB pathway proteins. (I, K) Colony-forming unit fibroblast (CFU-F) assay and quantification of self-renewal capacity (n = 3). (J) Senescence-associated β-galactosidase (SA-β-gal) activity. (L, M) Apoptosis analysis by Annexin V/PI flow cytometry and quantification (n = 3). Scale bars: 100 μm (C); 5 μm (D, G); 200 μm (J). Significance: ns, not significant; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.
Techniques Used: CCK-8 Assay, Immunofluorescence, Staining, Membrane, Western Blot, Activity Assay, Flow Cytometry
Related Articles
Western Blot:Article Title: Ginkgetin Alleviates Inflammation and Senescence by Targeting STING Article Snippet: The next day, the membranes were subsequently incubated for 2 h at RT with an appropriate secondary antibody and then incubated with ECL luminescence reagent (Meilunbio, MA0186) and visualized using GeneGnome XRQ NPC (Shanghai, China). .. The antibodies used for western blotting were as followed: anti‐p‐STING (Ser366) (50907S; 1:500), anti‐p‐STING (Ser365) (72971S; 1:500), anti‐STING (13647S; 1:1000), anti‐p‐TBK1 (Ser172) (5483S; 1:500), anti‐TBK1 (3504S; 1:1000), Article Title: Ginkgetin Alleviates Inflammation and Senescence by Targeting STING. Article Snippet: The next day, the membranes were subsequently incubated for 2 h at RT with an appropriate secondary antibody and then incubated with ECL luminescence reagent (Meilunbio, MA0186) and visualized using GeneGnome XRQ NPC (Shanghai, China). .. The antibodies used for western blotting were as followed: anti-pSTING (Ser366) (50907S; 1:500), anti-p-STING (Ser365) (72971S; 1:500), anti-STING (13647S; 1:1000), anti-p-TBK1 (Ser172) (5483S; 1:500), antiTBK1 (3504S; 1:1000), other:Article Title: VHL loss enhances antitumor immunity by activating the anti-viral DNA-sensing pathway Article Snippet: |